Apex1 can cleave complex clustered DNA lesions in cells
نویسندگان
چکیده
منابع مشابه
Unrepaired clustered DNA lesions induce chromosome breakage in human cells.
Clustered DNA damage induced by ionizing radiation is refractory to repair and may trigger carcinogenic events for reasons that are not well understood. Here, we used an in situ method to directly monitor induction and repair of clustered DNA lesions in individual cells. We showed, consistent with biophysical modeling, that the kinetics of loss of clustered DNA lesions was substantially comprom...
متن کاملOxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations
DNA damage and mutations induced by oxidative stress are associated with various different human pathologies including cancer. The facts that most human tumors are characterized by large genome rearrangements and glutathione depletion in mice results in deletions in DNA suggest that reactive oxygen species (ROS) may cause gene and chromosome mutations through DNA double strand breaks (DSBs). Ho...
متن کاملDNA gyrase can cleave short DNA fragments in the presence of quinolone drugs.
We have analysed the DNA cleavage reaction of DNA gyrase using oligonucleotides annealed to a single-stranded M13 derivative containing a preferred gyrase cleavage site. We find that gyrase can cleave duplexes down to approximately 20 bp in size in the presence of the quinolone drugs ciprofloxacin and oxolinic acid. Ciprofloxacin shows a variation in its site specificity with an apparent prefer...
متن کاملDNA repair of clustered lesions in mammalian cells: involvement of non-homologous end-joining
Clustered lesions are defined as >or=two lesions within 20 bps and are generated in DNA by ionizing radiation. In vitro studies and work in bacteria have shown that attempted repair of two closely opposed lesions can result in the formation of double strand breaks (DSBs). Since mammalian cells can repair DSBs by non-homologous end-joining (NHEJ), we hypothesized that NHEJ would repair DSBs form...
متن کاملClustered DNA damage leads to complex genetic changes in irradiated human cells.
Densely ionizing radiations interact with DNA to cause heavily clustered sites of damage that are difficult to repair correctly. We have been able to determine for the first time the breakpoints of several very large deletions induced by densely ionizing radiation in diploid human cells and show that damage clustering is reflected in the complexity of mutations. Intra- and interchromosomal inse...
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ژورنال
عنوان ژورنال: DNA Repair
سال: 2009
ISSN: 1568-7864
DOI: 10.1016/j.dnarep.2009.08.008